Brade V, Kreuzpaintner G
Immunobiology. 1982 Apr;161(3-4):315-21. doi: 10.1016/S0171-2985(82)80088-0.
In our studies on complement secretion functional C1, C4, C2, C3, P, D and B were clearly identified in the same cultures. Functional assays did not allow the detection of C5 to C9. Spontaneous C3 activation occurred at a very low level in culture supernatants. The responsible enzyme was identified as a metallo-enzyme. Upon addition of antibody-coated sheep erythrocytes (EA) to culture supernatant it was possible to induce C3 activation as indicated by the apparent formation of EAC1423. Zymosan was also able to activate C3 in culture supernatant after addition of purified functional factor B indicating efficient cooperation of factors of the alternative pathway. Thus in this in vitro system macrophages not only provide C3 but also all factors for spontaneous and induced C3 activation. If these secretory functions reflect in vivo properties of macrophages, our results may indicate that C3 and its activating systems are most relevant for local cooperation between macrophages and the complement system in inflammation and antimicrobial defense. Therefore availability of these essential factors at any time is secured by local production.
在我们对补体分泌功能的研究中，功能性C1、C4、C2、C3、P、D和B在同一培养物中得到了明确鉴定。功能测定无法检测到C5至C9。培养上清液中自发的C3激活水平非常低。相关酶被鉴定为金属酶。向培养上清液中加入抗体包被的绵羊红细胞（EA）后，能够诱导C3激活，表现为明显形成EAC1423。加入纯化的功能性因子B后，酵母聚糖也能够激活培养上清液中的C3，这表明替代途径的因子之间存在有效协作。因此，在这个体外系统中，巨噬细胞不仅提供C3，还提供自发和诱导C3激活所需的所有因子。如果这些分泌功能反映了巨噬细胞的体内特性，我们的结果可能表明，C3及其激活系统对于巨噬细胞与补体系统在炎症和抗菌防御中的局部协作最为重要。因此，这些必需因子可随时通过局部产生来确保供应。
