Buratti M, Calzaferri G, Caravelli G, Colombi A, Maroni M, Foà V
Int J Environ Anal Chem. 1984;17(1):25-34. doi: 10.1080/03067318408076965.
The aim of this research has been to develop analytical procedures whereby the various chemical forms of arsenic present in urine can be distinguished and further data on the biotransformation of absorbed arsenic can be acquired. The separation of inorganic arsenic ( InAs ), monomethylarsonic acid ( MMAA ), and dimethylarsinic acid ( DMAA ) in urine was performed by ion-exchange chromatography on AG 50 W-X8 resin. Arsenic was then measured directly on the eluted fractions by atomic absorption spectrophotometry, after the reduction of arsenic to the correspondent arsine. In 160 subjects with no occupational exposure to arsenic compounds, InAs , MMAA , DMAA each accounted for about 10% of the total arsenic urinary excretion (17.2 +/- 11.1 micrograms/1), thus indicating that in the normal population over 60% of arsenic in urine is present in other organic forms. After eating marine food, there was a marked increase of urinary output of arsenic, but no increase was observed in InAs , MMAA and DMAA urinary excretion. In the biological monitoring of exposure to inorganic arsenic, particularly in the case of high urinary excretion values, the differentiation of the excreted forms of arsenic is necessary to establish with certainty the source (industrial or alimentary) of arsenic.
本研究的目的是开发分析方法，借此区分尿液中砷的各种化学形态，并获取有关吸收砷生物转化的更多数据。尿液中无机砷（InAs）、一甲基胂酸（MMAA）和二甲基胂酸（DMAA）的分离通过在AG 50 W-X8树脂上进行离子交换色谱法完成。在将砷还原为相应的胂后，通过原子吸收分光光度法直接对洗脱组分中的砷进行测定。在160名无砷化合物职业暴露的受试者中，InAs、MMAA、DMAA分别约占尿砷总排泄量的10%（17.2±11.1微克/升），这表明在正常人群中，超过60%的尿砷以其他有机形态存在。食用海产品后，尿砷排出量显著增加，但InAs、MMAA和DMAA的尿排泄量未见增加。在对无机砷暴露进行生物监测时，特别是在尿排泄值较高的情况下，区分砷的排泄形态对于确切确定砷的来源（工业或饮食）是必要的。
