Takazono I, Tanaka Y
J Chromatogr. 1984 Apr 20;288(1):167-76. doi: 10.1016/s0021-9673(01)93691-x.
The separation and quantitative analysis of streptomyces hyaluronidase ( HAase ) degradation products of hyaluronic acid (HA) by high-performance liquid chromatography are described. The substituted 4,5-unsaturated tetrasaccharide and hexasaccharide which result from the digestion of HA are quickly separated on a silica gel (Zorbax SIL) column. The assay of HA is linear between 5 and 250 micrograms of HA. This procedure is suitable for some weakly acidic glycosaminoglycan contaminants having similar properties to those of HA.
本文描述了通过高效液相色谱法对透明质酸（HA）的链霉菌透明质酸酶（HAase）降解产物进行分离和定量分析的方法。由HA消化产生的取代4,5-不饱和四糖和六糖在硅胶（Zorbax SIL）柱上能快速分离。HA的测定在5至250微克HA之间呈线性关系。该方法适用于一些与HA具有相似性质的弱酸性糖胺聚糖污染物。
