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Determination of molecular weight of hyaluronic acid in bovine vitreous humour and Healon by high performance gel permeation chromatography and its depolymerization with ascorbic acid.

作者信息

Iqbal Z, Midgley J M, Watson D G, Karditsas S D, Dutton G N, Wilson W

机构信息

Department of Pharmacy, University of Strathclyde, Glasgow, U.K.

出版信息

Pharm World Sci. 1997 Oct;19(5):246-50. doi: 10.1023/a:1008644400293.

DOI:10.1023/a:1008644400293
PMID:9368926
Abstract

A method has been developed for the determination of the molecular weight of hyaluronic acid by high performance gel permeation chromatography. The molecular weight distribution was determined using three polymeric columns. Columns were calibrated using Pullulan Polymer molecular weight standards. The average molecular weight (+/- SD) of the hyaluronic acid in bovine vitreous humour and Healon were 1.20 x 10(6) Da (+/- 0.49 x 10(6) and 3.01 x 10(6) Da (+/- 0.14 x 10(6), respectively. Furthermore, the depolymerization of the hyaluronic acid in the presence of ascorbic acid (the concentration found in human eye) was studied. Samples incubated in the presence of atmospheric air showed more depolymerization than those where atmospheric air had been flushed-out with nitrogen.

摘要

相似文献

1
Determination of molecular weight of hyaluronic acid in bovine vitreous humour and Healon by high performance gel permeation chromatography and its depolymerization with ascorbic acid.
Pharm World Sci. 1997 Oct;19(5):246-50. doi: 10.1023/a:1008644400293.
2
The molecular weight of hyaluronate in the aqueous humour and vitreous body of rabbit and cattle eyes.兔眼和牛眼房水及玻璃体中透明质酸盐的分子量。
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Metal ion catalyzed liquefaction of vitreous by ascorbic acid: role of radicals and radical ions.
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Measurements of reducing end groups on bovine vitreous-humour hyaluronic acid by reaction with [14C]cyanide.通过与[14C]氰化物反应测定牛玻璃体液透明质酸上的还原端基。
Biochem J. 1982 Dec 1;207(3):409-14. doi: 10.1042/bj2070409.
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The inability of superoxide dismutase to inhibit the depolymerization of hyaluronic acid by ferrous ions and ascorbate.超氧化物歧化酶无法抑制亚铁离子和抗坏血酸介导的透明质酸解聚。
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;214(3):181-5. doi: 10.1007/BF00414560.
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FINGERPRINTING THE HYALURONIC ACID-PROTEIN COMPLEX OF HUMAN VITREOUS HUMOUR.
Clin Chim Acta. 1965 Feb;11:139-45. doi: 10.1016/0009-8981(65)90208-1.
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Jpn J Ophthalmol. 1997 May-Jun;41(3):154-9. doi: 10.1016/s0021-5155(97)00033-6.
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Improving the accuracy of hyaluronic acid molecular weight estimation by conventional size exclusion chromatography.通过传统尺寸排阻色谱法提高透明质酸分子量估计的准确性。
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Effects of hyaluronan lyase, hyaluronidase, and chondroitin ABC lyase on mammalian vitreous gel.透明质酸裂解酶、透明质酸酶和硫酸软骨素ABC裂解酶对哺乳动物玻璃体凝胶的作用。
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本文引用的文献

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6
Replacement of the liquid vitreus with sodium hyaluronate in monkeys. I. Short-term evaluation.在猴子中用透明质酸钠替代液体玻璃体。I. 短期评估。
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The molecular weight of hyaluronate in the aqueous humour and vitreous body of rabbit and cattle eyes.兔眼和牛眼房水及玻璃体中透明质酸盐的分子量。
Exp Eye Res. 1983 Apr;36(4):481-92. doi: 10.1016/0014-4835(83)90042-8.
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Hyaluronate in human aqueous humor.人房水中的透明质酸盐。
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9
Quantitative analysis of hyaluronic acid by high-performance liquid chromatography of streptomyces hyaluronidase digests.通过链霉菌透明质酸酶消化产物的高效液相色谱法对透明质酸进行定量分析。
J Chromatogr. 1984 Apr 20;288(1):167-76. doi: 10.1016/s0021-9673(01)93691-x.
10
Biochemical studies on the use of sodium hyaluronate in the anterior eye segment. II. The molecular behavior of sodium hyaluronate injected into anterior chamber of rabbits.透明质酸钠在前房的应用的生化研究。II. 注入兔前房的透明质酸钠的分子行为
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