Baba S, Utoh M, Horie M, Mori Y
J Chromatogr. 1984 Apr 13;307(1):1-9.
To estimate the 5-hydroxytryptamine level in human plasma by gas chromatography--mass spectrometry--selected ion monitoring (GC--MS--SIM), a recovery method from plasma and derivatization conditions with pentafluoropropionic anhydride were investigated. By heating 5-hydroxytryptamine at 140 degrees C for 4 h in the presence of pentafluoropropionic anhydride the peak intensity of derivatized 5-hydroxytryptamine increased more than three times in comparison with the reported method. There was a marked difference in the plasma levels of 5-hydroxytryptamine obtained using the GC--MS--SIM method compared to those obtained using fluorometric assay.
为了通过气相色谱-质谱联用-选择离子监测（GC-MS-SIM）法估算人血浆中的5-羟色胺水平，研究了从血浆中回收的方法以及使用五氟丙酸酐的衍生化条件。在五氟丙酸酐存在下，将5-羟色胺于140℃加热4小时，与报道的方法相比，衍生化的5-羟色胺的峰强度增加了三倍多。与使用荧光测定法获得的结果相比，使用GC-MS-SIM法获得的5-羟色胺血浆水平存在显著差异。
