Allender W J, Archer A W
J Forensic Sci. 1984 Apr;29(2):515-26.
A method is described for the determination of promethazine and some of its major metabolites in postmortem specimens by enzymic digestion followed by high-pressure liquid chromatography of the extracts using a cyano-bonded (mu-Bondapak-CN) silica column packing and a mobile phase consisting of methanol, water, and n-octylamine (adjusted to pH 8). The system will separate and quantitate promethazine sulphoxide, desmonomethyl promethazine, didesmethyl promethazine, and promethazine. Pericyazine (2-cyano-10-[3-(4- hydroxypiperidino )propyl] phenothiazine) was used as internal standard. The parent drug and metabolites were extracted from a enzyme digested tissue homogenates with ethyl acetate using a simple, single micro-extraction procedure. The method was applied to four cases of fatal poisoning involving promethazine ingestion.
本文描述了一种通过酶消化，接着对提取物进行高压液相色谱分析来测定死后标本中异丙嗪及其一些主要代谢物的方法。该色谱分析使用氰基键合（μ-Bondapak-CN）硅胶柱填料和由甲醇、水及正辛胺（调节至pH 8）组成的流动相。该系统可分离并定量异丙嗪亚砜、去甲异丙嗪、双去甲异丙嗪和异丙嗪。奋乃静（2-氰基-10-[3-(4-羟基哌啶基)丙基]吩噻嗪）用作内标。采用简单的单次微量萃取程序，用乙酸乙酯从酶消化的组织匀浆中提取母体药物及其代谢物。该方法应用于4例涉及异丙嗪摄入的致命中毒病例。
