Sone S, Tachibana K, Shono M, Ogushi F, Tsubura E
J Biol Response Mod. 1984;3(2):185-94.
Potentiation of the tumoricidal activity of human alveolar macrophages (AM) by muramyl dipeptide (MDP) and MDP in liposomes (liposome-MDP) was examined. Significant increase in AM-mediated cytotoxicity against allogeneic melanoma cells required the interaction of AM with liposome-MDP for a minimum of 4 h. The uptake by human AM of liposomes containing fluorescent quinacrine was examined. A linear correlation was found between the amount of liposomes added to AM monolayers and their phagocytosis of liposome-entrapped quinacrine. Addition of phosphatidylserine to liposomes composed of phosphatidylcholine enhanced both the phagocytosis of liposomes and the ability of liposome-MDP to potentiate the tumoricidal activity of human AM. In experiments on the dose response of liposomes containing 10 micrograms/ml MDP, 50 nmol of liposomes/10(5) AM caused maximal activation of human AM, and greater than 100 nmol of liposomes was less effective. These results show that multilamellar vesicle liposomes containing MDP are more effective than free MDP in potentiating the tumoricidal activity of human AM during culture for 4 h.
研究了胞壁酰二肽（MDP）和脂质体包裹的MDP（脂质体-MDP）对人肺泡巨噬细胞（AM）杀肿瘤活性的增强作用。AM介导的对同种异体黑色素瘤细胞的细胞毒性显著增加，要求AM与脂质体-MDP相互作用至少4小时。检测了人AM对含荧光喹吖因的脂质体的摄取。在添加到AM单层的脂质体数量与其对脂质体包裹的喹吖因的吞噬作用之间发现了线性相关性。向由磷脂酰胆碱组成的脂质体中添加磷脂酰丝氨酸，增强了脂质体的吞噬作用以及脂质体-MDP增强人AM杀肿瘤活性的能力。在含10微克/毫升MDP的脂质体的剂量反应实验中，50纳摩尔脂质体/10⁵个AM可使人AM产生最大激活，而大于100纳摩尔脂质体的效果则较差。这些结果表明，含MDP的多层囊泡脂质体在培养4小时期间增强人AM杀肿瘤活性方面比游离MDP更有效。
