Zamir N, Palkovits M, Brownstein M J
J Neurosci. 1984 May;4(5):1240-7. doi: 10.1523/JNEUROSCI.04-05-01240.1984.
Using a specific radioimmunoassay, we have found that immunoreactive (ir) alpha-neo-endorphin has a widespread and unique distribution in the rat brain and spinal cord. The highest concentration in brain is in the substantia nigra (1692.1 fmol/mg of protein). Very high concentrations of ir-alpha- neo-endorphin (greater than 500 fmol/mg of protein) are also found in the lateral preoptic nucleus, dentate gyrus, parabrachial nuclei, nucleus accumbens, globus pallidus, median eminence, and anterior hypothalamic nucleus. Relatively high concentrations of ir-alpha-neo-endorphin (250 to 500 fmol/mg of protein) are present in the bed nucleus of the stria terminalis, paraventricular nucleus, nucleus of the solitary tract, dorsomedial nucleus, central amygdaloid nucleus, periaqueductal gray matter, suprachiasmatic nucleus, periventricular nucleus, hippocampus, prepositus hypoglossal nucleus, arcuate nucleus, ventromedial nucleus, medial preoptic nucleus, zona incerta, dorsal premamillary nucleus, medial forebrain bundle (hypothalamic and preoptic), nucleus of the diagonal band, locus ceruleus, lateral septal nucleus, and nucleus ambiguus . Moderate levels (100 to 250 fmol/mg of protein) are found in the posterior hypothalamic nucleus, ventral premamillary nucleus, dorsal raphe nucleus, motor hypoglossal nucleus, caudate-putamen, periventricular thalamic nucleus, subcommissural organ, sensory trigeminal nucleus, perifornical nucleus, area postrema, supraoptic nucleus, cuneate nucleus, medial amygdaloid nucleus, and organum vasculosum laminae terminalis. Low concentrations of ir-alpha-neo-endorphin (less than 100 fmol/mg of protein) are found in many cortical structures, claustrum, thalamic nuclei, habenular nuclei, lateral geniculate body, red nucleus, superior and inferior colliculi, paramedian reticular nucleus, pontine nuclei, superior olive, vestibular nuclei, motor facial nucleus, gigantocellular reticular nucleus, and subfornical organ.(ABSTRACT TRUNCATED AT 250 WORDS)
通过一种特定的放射免疫测定法，我们发现免疫反应性（ir）α-新内啡肽在大鼠脑和脊髓中分布广泛且独特。脑中浓度最高的部位是黑质（1692.1飞摩尔/毫克蛋白质）。视前外侧核、齿状回、臂旁核、伏隔核、苍白球、正中隆起和下丘脑前核中也发现了非常高浓度的ir-α-新内啡肽（大于500飞摩尔/毫克蛋白质）。终纹床核、室旁核、孤束核、背内侧核、中央杏仁核、导水管周围灰质、视交叉上核、室周核、海马、舌下前置核、弓状核、腹内侧核、视前内侧核、未定带、背侧乳头前核、内侧前脑束（下丘脑和视前区）、斜角带核、蓝斑、外侧隔核和疑核中存在相对较高浓度的ir-α-新内啡肽（250至500飞摩尔/毫克蛋白质）。下丘脑后核、腹侧乳头前核、背侧中缝核、舌下运动核、尾状核-壳核、室周丘脑核、连合下器官、感觉三叉神经核、穹窿周核、最后区、视上核、楔状核、内侧杏仁核和终板血管器中发现中等水平（100至250飞摩尔/毫克蛋白质）。在许多皮质结构、屏状核、丘脑核、缰核、外侧膝状体、红核、上丘和下丘、旁正中网状核、脑桥核、上橄榄核、前庭核、面神经运动核、巨细胞网状核和穹窿下器官中发现低浓度的ir-α-新内啡肽（小于100飞摩尔/毫克蛋白质）。（摘要截短于250词）
