Chigaleĭchik A G, Vinogradov B D, Orlova V S, Rylkin S S
Mikrobiologiia. 1978 May-Jun;47(3):409-13.
The activity of deamidases of L-glutamine and L-asparagine of Pseudomonas boreopolis 526 was found to depend on the conditions of cultivation. The activity of L-glutaminase-asparaginase II (EC 3.5.1.38) decreased with an increase of the specific growth rate in the conditions of periodic cultivation and increased in the conditions of continuous cultivation. The activity of this enzyme was manifested mainly in the conditions of continuous cultivation at a rate of 0.1--0.2 hr-1 and a final nitrogen concentration of 0.006--0.025 g per litre of the medium. If the concentration of nitrogen was increased in these conditions, biosynthesis of L-glutaminase-asparaginase I was stimulated while the activity of L-glutaminase-asparaginase II was not detected at all.
已发现极地假单胞菌526的L-谷氨酰胺和L-天冬酰胺脱酰胺酶的活性取决于培养条件。在分批培养条件下，L-谷氨酰胺酶-天冬酰胺酶II（EC 3.5.1.38）的活性随比生长速率的增加而降低，而在连续培养条件下则增加。该酶的活性主要在连续培养条件下表现出来，培养速率为0.1 - 0.2小时-1，培养基中最终氮浓度为每升0.006 - 0.025克。如果在这些条件下增加氮浓度，则会刺激L-谷氨酰胺酶-天冬酰胺酶I的生物合成，而根本检测不到L-谷氨酰胺酶-天冬酰胺酶II的活性。
