[L-glutaminase-asparaginase biosynthesis by a Pseudomonas boreopolis culture dependent on cultivation conditions].

The activity of deamidases of L-glutamine and L-asparagine of Pseudomonas boreopolis 526 was found to depend on the conditions of cultivation. The activity of L-glutaminase-asparaginase II (EC 3.5.1.38) decreased with an increase of the specific growth rate in the conditions of periodic cultivation and increased in the conditions of continuous cultivation. The activity of this enzyme was manifested mainly in the conditions of continuous cultivation at a rate of 0.1--0.2 hr-1 and a final nitrogen concentration of 0.006--0.025 g per litre of the medium. If the concentration of nitrogen was increased in these conditions, biosynthesis of L-glutaminase-asparaginase I was stimulated while the activity of L-glutaminase-asparaginase II was not detected at all.