Proops D W, Hawke W M, Parkinson E K
J Otolaryngol. 1984 Apr;13(2):63-9.
There has been considerable research interest in cholesteatoma in recent years but an understanding of the pathology has been handicapped by the lack of a suitable research model. Animal experiments are unsatisfactory as they may not fully represent the human pathology. In 1975 Rheinwald and Green devised a method of growing skin in tissue culture, using a feeder layer of lethally irradiated fetal mouse fibroblasts. This technique has been adapted for use with otological surgical specimens so that a comparison can be made between skin colonies derived from cholesteatoma matrix, migratory epithelium of the external ear, and normal skin from an extraconchal incision, as well as foreskin from neonatal circumcision as a control. The colonies so produced are then available for study by phase contrast microscopy, conventional light microscopy, and scanning and transmission electron microscopy. Differences in behavior and morphology between the various colonies grown in tissue culture have been demonstrated.
近年来，胆脂瘤引起了相当大的研究兴趣，但由于缺乏合适的研究模型，对其病理学的理解受到了阻碍。动物实验并不令人满意，因为它们可能无法完全代表人类病理学情况。1975年，莱茵瓦尔德和格林设计了一种在组织培养中培养皮肤的方法，使用经致死剂量照射的胎鼠成纤维细胞作为饲养层。这项技术已被改编用于耳科手术标本，以便对源自胆脂瘤基质、外耳道迁移上皮、耳郭外切口的正常皮肤以及新生儿包皮环切术的包皮作为对照所产生的皮肤菌落进行比较。然后可以通过相差显微镜、传统光学显微镜以及扫描和透射电子显微镜对所产生的菌落进行研究。已经证明了在组织培养中生长的各种菌落之间在行为和形态上的差异。
