Vani B R, Ramakrishnan T, Kuchino Y, Nishimura S
Nucleic Acids Res. 1984 May 11;12(9):3933-6. doi: 10.1093/nar/12.9.3933.
The nucleotide sequence of initiator tRNA from Mycobacterium smegmatis was determined to be pCGCGGGGUGGAGCAGCUCGGDAGCUCGCUGGGCUCAUAACCCAGAGm7GUCG CAGGU psi CGm1AAUCCUGUCCCCGCUACCAOH . The nucleotide sequence of Mycobacterium initiator tRNA was found to be the same as that of Streptomyces initiator tRNA, except that G46 and A57 were replaced by m7G46 and G57 , respectively. The striking feature of Mycobacterium initiator tRNA is the absence of ribothymidine at residue 54, and the presence of 1-methyladenosine at residue 58 which makes the sequence of this tRNA similar to that of eukaryotic initiator tRNA.
耻垢分枝杆菌起始tRNA的核苷酸序列被确定为pCGCGGGGUGGAGCAGCUCGGDAGCUCGCUGGGCUCAUAACCCAGAGm7GUCG CAGGUψCGm1AAUCCUGUCCCCGCUACCAOH。发现分枝杆菌起始tRNA的核苷酸序列与链霉菌起始tRNA的序列相同,只是G46和A57分别被m7G46和G57取代。分枝杆菌起始tRNA的显著特征是在第54位残基处没有核糖胸腺嘧啶,而在第58位残基处有1-甲基腺苷,这使得该tRNA的序列与真核生物起始tRNA的序列相似。