Kuchino Y, Mita T, Nishimura S
Nucleic Acids Res. 1981 Sep 25;9(18):4557-62. doi: 10.1093/nar/9.18.4557.
The total primary structure of cytoplasmic initiator tRNA from Tetrahymena thermophila mating type IV, was determined by post labeling techniques. The sequence is pa-G-C-A-G-G-G-U-m1G-G-C-G-A-A-A-D-Gm-G-A-A-U-C-G-C-G-U-Psi-G-G-G-C-U-C-A-U-t6A -A-C-Psi-C-A-A-A-A-m7G-U-m5C-A-G-A-G-G-A-Psi-C-G-m1A-A-A-C-C-U-C-U-C-U-C-U-G-C- U-A-C-C-AOH. The nucleotide residue in the position next to the 5'-end of the anticodon of this tRNA (residue No. 33) is uridine instead of cytidine, which has been found in cytoplasmic initiator tRNAs from multicellular eukaryotic organisms. The sequence of three consecutive G-C base pairs in the anticodon stem common to all other cytoplasmic initiator tRNAs is disrupted in this tRNA; namely, the cytidine at residue 40 in this region is replaced by pseudouridine in Tetrahymena initiator tRNA.
嗜热四膜虫IV型交配型细胞质起始tRNA的完整一级结构是通过后标记技术确定的。其序列为pa-G-C-A-G-G-G-U-m1G-G-C-G-A-A-A-D-Gm-G-A-A-U-C-G-C-G-U-Psi-G-G-G-C-U-C-A-U-t6A -A-C-Psi-C-A-A-A-A-m7G-U-m5C-A-G-A-G-G-A-Psi-C-G-m1A-A-A-C-C-U-C-U-C-U-C-U-G-C- U-A-C-C-AOH。该tRNA反密码子5'端相邻位置的核苷酸残基（第33号残基）是尿苷，而非胞苷，在多细胞真核生物的细胞质起始tRNA中发现的是胞苷。该tRNA中所有其他细胞质起始tRNA共有的反密码子茎中的三个连续G-C碱基对序列被破坏；即，该区域第40号残基处的胞苷在嗜热四膜虫起始tRNA中被假尿苷取代。
