Zakaria A H, Sakai H, Imai K
Poult Sci. 1984 May;63(5):1061-3. doi: 10.3382/ps.0631061.
To demonstrate time of transformation of ovarian follicles from the resting stage to the rapid growth phase, Sudan black in ethanol was intravenously injected into laying hens, and 4 hr later Sudan red in water was injected into the same hens; the hens were killed 4 hr after the last injection for observation of the follicles. Injections were made at various times during an ovulatory cycle. In an additional experiment, hourly yolk deposition in the dark period (1900 to 0500 hr) was compared with that in the light period. Follicular transformation occurred at a high frequency within the period of 8 to 14 hr after ovulation. This period corresponded to the time between 1700 and 2400 hr. Hourly yolk deposition in the dark period was higher than that in the light period.
为了证明卵巢卵泡从静止期转变为快速生长阶段的时间，将乙醇中的苏丹黑静脉注射到产蛋母鸡体内，4小时后将水中的苏丹红注射到同一只母鸡体内；在最后一次注射后4小时处死母鸡以观察卵泡。在排卵周期的不同时间进行注射。在另一项实验中，比较了黑暗期（19:00至05:00）和光照期每小时的卵黄沉积情况。卵泡转变在排卵后8至14小时内高频发生。这段时间对应于17:00至24:00之间的时间段。黑暗期每小时的卵黄沉积高于光照期。
