Himanen J P, Elo A, Korpela T
Prog Clin Biol Res. 1984;144A:109-14.
Convenient chromatographic methods to measure equilibrium constants between cyclodextrins and aromatic compounds were developed. The use of 'Hummel-Dreyer' elution profile allows us to determine values above 15-20 M-1. The weaker associations (below 15-20 M-1) can be measured with cyclo-dextrin-derivatized Biogel P-2 column. The latter method is very suitable to determine the equilibrium constants between cyclodextrins and vitamin B6-compounds which can't be measured with the conventional photometric procedure.
开发了用于测量环糊精与芳香族化合物之间平衡常数的便捷色谱方法。使用“Hummel-Dreyer”洗脱曲线使我们能够测定高于15 - 20 M⁻¹的值。较弱的缔合作用（低于15 - 20 M⁻¹）可用环糊精衍生化的Biogel P - 2柱进行测量。后一种方法非常适合测定环糊精与维生素B6化合物之间的平衡常数，而这是用传统光度法无法测量的。
