Nakatani H, Hiromi K
J Biochem. 1984 Jul;96(1):69-72. doi: 10.1093/oxfordjournals.jbchem.a134830.
The pressure dependence of the formation and dissociation rate constants of the inclusion reaction between beta-cyclodextrin and phenolphthalein was investigated with a newly constructed high-pressure temperature-jump apparatus. The jump height of temperature was 2.8 degrees C within 1 microsecond under pressure up to 2,000 kg/cm2. The reaction was analyzed as a simple reversible bimolecular reaction. The activation volume was 1.78 +/- 0.85 cm3/mol for the forward reaction and 10.65 +/- 1.40 cm3/mol for the backward reaction. The values of activation volumes suggest that desolvation from the phenolphthalein molecule and release of hydrating water molecules from the beta-cyclodextrin cavity occur at the transition state.
利用新构建的高压温度跃升装置，研究了β-环糊精与酚酞包合反应的形成和解离速率常数与压力的关系。在高达2000 kg/cm²的压力下，温度跃升高度在1微秒内为2.8℃。该反应被分析为简单的可逆双分子反应。正向反应的活化体积为1.78±0.85 cm³/mol，逆向反应的活化体积为10.65±1.40 cm³/mol。活化体积的值表明，在过渡态时，酚酞分子发生去溶剂化，β-环糊精腔内的水化水分子被释放。
