Ware J A, Horak J K, Bolli R, Mathur V S, Massumi G A, Solis R T
Thromb Res. 1984 Apr 1;34(1):9-18. doi: 10.1016/0049-3848(84)90102-6.
To investigate in vivo and in vitro microaggregation in coronary artery disease, we obtained blood samples from the coronary sinus (CS), pulmonary artery (PA), and aorta (AO) in patients undergoing cardiac catheterization. An electronic particle size analyzer was used to quantify microaggregates 13 to 81 mu in diameter in blood. In the first group of 58 patients, preformed circulating microaggregates and platelet responsiveness to ADP were assessed in AO and PA blood only. The coronary artery disease patients did not have significantly higher volumes of preformed in vivo aggregates in either AO or PA blood. However, the mean aggregate size in response to 0.2 microM ADP in vitro was larger in both AO and PA blood in patients with coronary disease [12.4 +/- 0.9 vs. 9.4 +/- 1.4 X 10(3) mu3 (AO); 12.5 +/- 0.9 vs. 8.3 +/- 0.7 0.7 X 10(3) mu3 (PA)]. In a second group of 46 patients, CS, AO and PA samples were compared using the same methods. The volume of microaggregates preformed in vivo was significantly greater in CS blood than in PA or AO blood in patients with and without coronary disease. The volume and mean size of aggregates induced by ADP in vitro were smaller in CS blood compared to PA. In conclusion, the volume of in vivo microaggregates is increased in CS blood, independent of coronary disease, but significant volumes are not found in PA or AO blood. Patients with coronary disease have more reactive platelets to in vitro aggregatory agents in AO and PA samples of similar hematocrit.
为了研究冠心病患者体内和体外的微聚集情况，我们从接受心导管检查的患者的冠状静脉窦（CS）、肺动脉（PA）和主动脉（AO）采集了血样。使用电子粒度分析仪对血液中直径为13至81微米的微聚集体进行定量分析。在第一组58例患者中，仅对AO和PA血液中的预先形成的循环微聚集体和血小板对ADP的反应性进行了评估。冠心病患者的AO或PA血液中预先形成的体内聚集体体积均无显著增加。然而，冠心病患者的AO和PA血液中，对0.2微摩尔/升ADP体外反应的平均聚集体大小更大[12.4±0.9对9.4±1.4×10³立方微米（AO）；12.5±0.9对8.3±0.7×10³立方微米（PA）]。在第二组46例患者中，使用相同方法比较了CS、AO和PA样本。无论有无冠心病，CS血液中体内预先形成的微聚集体体积均显著大于PA或AO血液中的。与PA相比，CS血液中ADP体外诱导的聚集体体积和平均大小较小。总之，CS血液中体内微聚集体体积增加，与冠心病无关，但在PA或AO血液中未发现显著体积增加。冠心病患者在血细胞比容相似的AO和PA样本中，其血小板对体外聚集剂的反应性更高。
