Clay M, Kline W, McCullough J
Transfusion. 1984 May-Jun;24(3):252-5. doi: 10.1046/j.1537-2995.1984.24384225033.x.
We examined sera from 2313 postpartum women as a potential source of granulocyte specific antibodies. Lymphocyte cytotoxic (LC) antibodies were detected in 397 (17.2%) specimens and granulocyte agglutinating (GA) antibodies in 291 (12.6%). Only two GA positive sera had reactivity for previously defined granulocyte specific antigens (one NA1 and NB1 ). One additional serum had GA reactivity similar to a serum previously reported by van Rood and called anti-6B. The frequency of granulocyte specific antibodies (0.1 percent) in our study indicates that the productivity of random screening of parous sera for granulocyte specific antibodies is low. A four-generation family study of the 6B antigen demonstrated a parallel association between HLA-B7 and B40 and 6B reactivity in the LC, GA, and granulocyte immunofluorescence (GIF) assay. This illustrates that the granulocyte reactivity of anti-6B is not granulocyte specific but is HLA related. This report provides additional evidence that HLA antibodies such as anti-B7 (6B) can react with granulocytes bearing these antigens in the GA and GIF assays currently used to identify granulocyte specific antibodies.
我们检测了2313名产后女性的血清，将其作为粒细胞特异性抗体的潜在来源。在397份（17.2%）标本中检测到淋巴细胞毒性（LC）抗体，在291份（12.6%）标本中检测到粒细胞凝集（GA）抗体。只有两份GA阳性血清对先前定义的粒细胞特异性抗原有反应性（一份NA1和一份NB1）。另外一份血清具有与van Rood先前报道的一份血清相似的GA反应性，该血清被称为抗-6B。我们研究中粒细胞特异性抗体的频率（0.1%）表明，对经产血清进行粒细胞特异性抗体随机筛查的效率很低。对6B抗原的四代家系研究表明，在LC、GA和粒细胞免疫荧光（GIF）试验中，HLA - B7和B40与6B反应性之间存在平行关联。这说明抗-6B的粒细胞反应性并非粒细胞特异性的，而是与HLA相关。本报告提供了更多证据，表明在目前用于鉴定粒细胞特异性抗体的GA和GIF试验中，诸如抗-B7（6B）等HLA抗体可与携带这些抗原的粒细胞发生反应。
