绵羊腐蹄病致病菌——结节拟杆菌外膜蛋白分析

Analysis of the outer membrane proteins of Bacteroides nodosus, the causal organism of ovine footrot.

作者信息

Emery D L, Clark B L, Stewart D J, O'Donnell I J, Hewish D R

出版信息

Vet Microbiol. 1984 Apr;9(2):155-68. doi: 10.1016/0378-1135(84)90031-2.

Abstract

Examination by SDS-PAGE of lithium acetate extracts of several strains of depiliated Bacteroides nodosus revealed 6 major outer membrane proteins (including pilin). The 5 membrane proteins exhibited approximate molecular weights of 75000, 50000, 38000, 34500 and 26500 whereas pilin had a MW of 17500 for the majority of strains. All proteins were accessible to lactoperoxidase-catalysed iodination and proteins 1, 2 and 5 were shown to be glycoproteins. Several attempts to isolate individual OMC proteins in pure form by selective solubilization and gel filtration were unsuccessful, but electroelution of individual outer membrane complex proteins resolved by SDS-PAGE provided sufficient quantities of antigen for immunization of sheep and for immunochemical analysis.

摘要

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对几株脱纤毛结节拟杆菌的醋酸锂提取物进行检测，发现了6种主要的外膜蛋白（包括菌毛蛋白）。这5种膜蛋白的分子量约为75000、50000、38000、34500和26500，而大多数菌株的菌毛蛋白分子量为17500。所有蛋白质都可被乳过氧化物酶催化碘化，并且蛋白1、2和5被证明是糖蛋白。通过选择性溶解和凝胶过滤以纯形式分离单个外膜复合物蛋白的几次尝试均未成功，但通过SDS-PAGE分离的单个外膜复合物蛋白的电洗脱为绵羊免疫和免疫化学分析提供了足够量的抗原。