Emery D L, Stewart D J
Vet Microbiol. 1984 Apr;9(2):169-79. doi: 10.1016/0378-1135(84)90032-4.
Phagocytosis of Bacteroides nodosus by ovine peripheral blood leucocytes (PBL) was examined after organisms had been opsonized in sera from normal sheep, or from animals immune to, or infected with ovine footrot . Ingestion of bacteria, as assessed microscopically or by counting isotopically-labelled organisms spectrometrically was effected in suspensions by polymorphonuclear leucocytes (PMN). Opsonization of bacteria in immune serum, particularly its IgG2 isotype, enhanced the rate of phagocytosis by PMN compared with that promoted by normal serum or medium alone. Whereas IgG2 from immune serum also increased the rate of ingestion of B. nodosus by adherent PMN, IgM and IgG1 from immune serum also initiated phagocytosis of bacteria by adherent ovine monocytes. Leucocytes from normal, immune or infected sheep of different breeds ingested B. nodosus with equal facility.
在用正常绵羊血清、免疫绵羊血清或感染羊蹄腐病动物的血清对诺氏拟杆菌进行调理后，检测了绵羊外周血白细胞（PBL）对其的吞噬作用。通过显微镜观察或用光谱法计数同位素标记的微生物来评估细菌摄取情况，该摄取过程由多形核白细胞（PMN）在悬浮液中完成。与单独使用正常血清或培养基相比，免疫血清（尤其是其IgG2同种型）对细菌的调理作用增强了PMN的吞噬速率。虽然免疫血清中的IgG2也提高了黏附性PMN摄取诺氏拟杆菌的速率，但免疫血清中的IgM和IgG1也启动了黏附性绵羊单核细胞对细菌的吞噬作用。不同品种的正常、免疫或感染绵羊的白细胞摄取诺氏拟杆菌的能力相同。
