Demotes-Mainard F, Vinçon G, Jarry C, Albin H
Ann Biol Clin (Paris). 1984;42(1):9-13.
A plasma assay for paracetamol by HPLC is described. Paracetamol is extracted with diethyl ether in the presence of an internal standard, beta- hydroxyethyltheophylline . The extracts are analysed using reversed phase chromatography (Radial Pak C18 column), the mobile phase consisting of a buffer mixture of 0.01 mol/l acetate (pH 4)-acetonitrile (920-80, v/v). The method is simple, rapid, precise and sensitive (lower level of sensitivity 0.13 mumol/l). It was used in a pharmacokinetic study in twelve volunteers.
本文描述了一种采用高效液相色谱法测定血浆中对乙酰氨基酚的方法。在内部标准物β-羟乙茶碱存在的情况下，用乙醚萃取对乙酰氨基酚。萃取物采用反相色谱法（径向Pak C18柱）进行分析，流动相由0.01 mol/l乙酸盐（pH 4）-乙腈（920-80，v/v）的缓冲混合物组成。该方法简便、快速、精确且灵敏（最低检测限为0.13 μmol/l）。它被用于12名志愿者的药代动力学研究。
