Whelpton R, Fernandes K, Wilkinson K A, Goldhill D R
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
Biomed Chromatogr. 1993 Mar-Apr;7(2):90-3. doi: 10.1002/bmc.1130070209.
A simple, but sensitive, micro-assay for paracetamol in blood and plasma is described. Samples were prepared by precipitation with trichloroacetic assay solution and the drug quantified by reversed phase liquid chromatography with dual electrode electrochemical detection in the redox mode (oxidation at +0.25 V followed by reduction at -0.15 V). The limit of detection was 0.1 mg/L for a 10 microL sample of blood or plasma, the coefficient of variation at this concentration being 11.4%. The method was applied to a study of gastric emptying in patients before and after cardiac surgery.
本文描述了一种用于血液和血浆中扑热息痛的简单但灵敏的微量测定法。样品通过用三氯乙酸测定溶液沉淀来制备，药物通过反相液相色谱法在氧化还原模式下用双电极电化学检测进行定量（在+0.25 V氧化，随后在-0.15 V还原）。对于10微升血液或血浆样品，检测限为0.1毫克/升，该浓度下的变异系数为11.4%。该方法应用于心脏手术前后患者胃排空的研究。
