Hodges K B, Penny J E, Brown R D, Henley C M
Arch Otolaryngol. 1984 Jul;110(7):429-36. doi: 10.1001/archotol.1984.00800330011002.
This investigation was performed to determine the morphologic effects of bacterial otitis media on the organ of Corti. The middle ear cavities of rats were inoculated with Streptococcus pneumoniae or saline and the animals were killed on days 1, 4, 7, 10, 14, and 21 after inoculation. Middle ear cultures were obtained and the cochleas were examined using scanning electron microscopy. All animals killed on day 1 had positive cultures, but by day 21, all cultures were negative. Cochlear changes observed were (1) damage to supporting cells (Deiters' cells), (2) morphologic changes of hair cell stereocilia, and (3) loss of inner and outer hair cell stereocilia and cell bodies (to a lesser extent), especially in the lower middle and basal turns. These changes appeared to occur in a definite sequence; ie, damage to the supporting cells, changes in stereocilia, and, finally, hair cell loss. These data show that cochlear damage and hair loss can be associated with bacterial otitis media.
本研究旨在确定细菌性中耳炎对柯蒂氏器的形态学影响。给大鼠的中耳腔接种肺炎链球菌或生理盐水，并在接种后第1、4、7、10、14和21天处死动物。获取中耳培养物，并使用扫描电子显微镜检查耳蜗。在第1天处死的所有动物培养物均呈阳性，但到第21天，所有培养物均为阴性。观察到的耳蜗变化有：（1）支持细胞（Deiters细胞）受损；（2）毛细胞静纤毛的形态变化；（3）内、外毛细胞静纤毛和细胞体缺失（程度较轻），尤其在中下部和基部螺旋。这些变化似乎按一定顺序发生，即支持细胞受损、静纤毛变化，最后是毛细胞丢失。这些数据表明，耳蜗损伤和毛细胞丢失可能与细菌性中耳炎有关。
