Breinlich T, Giebel W
Arch Otorhinolaryngol. 1984;240(1):95-101. doi: 10.1007/BF00464352.
Frozen sections of cholesteatomas were compared with postauricular and auditory canal skin and studied using histochemical methods. Several dehydrogenases, lysosomal enzymes and proteolytic activity were studied. Lactate and malate dehydrogenase activity was very strong in the epithelium and subepithelial tissue of cholesteatomas; no succinate dehydrogenase activity was observed. Esterase and acid phosphatase activity was prominent in cells of granulation tissue. Considerable proteolytic activity was observed in these cells and one specimen showed possible extracellular activity. Strong evidence of fat was found in the granulation tissue, particularly at the interface of granulation tissue and bone. In postauricular and auditory canal skin, enzyme activity was generally weaker; no evidence of fat was found. The findings are discussed in the light of other investigations on the importance of enzyme activity in bone destruction associated with cholesteatoma.
对胆脂瘤的冰冻切片与耳后皮肤及耳道皮肤进行比较，并采用组织化学方法进行研究。研究了几种脱氢酶、溶酶体酶和蛋白水解活性。乳酸脱氢酶和苹果酸脱氢酶活性在胆脂瘤的上皮和上皮下组织中非常强；未观察到琥珀酸脱氢酶活性。酯酶和酸性磷酸酶活性在肉芽组织细胞中很突出。在这些细胞中观察到相当强的蛋白水解活性，并且一个标本显示可能存在细胞外活性。在肉芽组织中发现了大量脂肪的有力证据，特别是在肉芽组织与骨的界面处。在耳后和耳道皮肤中，酶活性通常较弱；未发现脂肪的证据。结合其他关于酶活性在与胆脂瘤相关的骨质破坏中的重要性的研究对这些发现进行了讨论。
