Aberg B, Bagger-Sjöbäck D, Edström S, Heyden G, Jacobsson M, Kindblom L G
Department of Otolaryngology, Sahlgrenska Sjukhuset, University of Göteborg, Sweden.
Acta Otolaryngol. 1990 Jan-Feb;109(1-2):130-6. doi: 10.3109/00016489009107424.
Spontaneous and experimentally induced cholesteatoma in the Mongolian gerbil has been found to exhibit histopathological similarities to human aural cholesteatoma and has been suggested as an experimental model for studies of the clinical situation. In an attempt to further characterize this model, we compared experimentally induced cholesteatomas in the external auditory canal from gerbils with those of the human ear by means of a correlated histopathologic and enzyme histochemical study. The human and gerbilline cholesteatomas revealed similar histopathologic features. Even enzyme histochemically, the human and experimentally induced cholesteatomas demonstrated similar features. Thus glucose-6-phosphate dehydrogenase activity, an indicator of oxidative metabolism, was demonstrated especially in the stratum granulosum cells of the heavily orthokeratinizing squamous epithelium adjacent to the cholesteatomas. The human ear canal skin also revealed enzyme histochemical characteristics similar to the squamous epithelium lining the human cholesteatoma. The hydrolytic enzyme activity (leucyl-aminopeptidase) was strong in the connective tissue surrounding human cholesteatoma when compared with that of ear canal skin. In the gerbilline cholesteatoma, this activity was demonstrated especially in the connective tissue adjacent to eroded bone, which possibly may facilitate cholesteatoma progression. We conclude that experimentally induced cholesteatoma has both histophatological and enzyme histochemical similarities to human aural cholesteatoma and therefore it is suggested that the gerbilline model may be used for studies on the development of human cholesteatoma. Our results support the view that cholesteatoma may originate from migrated hyperkeratinizing cells from the epidermis of the tympanic membrane or the meatus.
已发现蒙古沙鼠自发形成的和实验诱导形成的胆脂瘤在组织病理学上与人耳胆脂瘤相似，并被提议作为研究临床情况的实验模型。为了进一步描述该模型的特征，我们通过相关的组织病理学和酶组织化学研究，比较了沙鼠外耳道实验诱导形成的胆脂瘤与人耳胆脂瘤。人和沙鼠的胆脂瘤显示出相似的组织病理学特征。甚至在酶组织化学方面，人和实验诱导形成的胆脂瘤也表现出相似的特征。因此，作为氧化代谢指标的葡萄糖-6-磷酸脱氢酶活性，尤其在与胆脂瘤相邻的高度正角化鳞状上皮的颗粒层细胞中得以显示。人耳道皮肤在酶组织化学特征上也与人胆脂瘤内衬的鳞状上皮相似。与人耳道皮肤相比，人胆脂瘤周围结缔组织中的水解酶活性（亮氨酰氨基肽酶）较强。在沙鼠胆脂瘤中，这种活性尤其在与侵蚀骨相邻的结缔组织中得以显示，这可能促进胆脂瘤的进展。我们得出结论，实验诱导形成的胆脂瘤在组织病理学和酶组织化学上与人耳胆脂瘤均相似，因此建议沙鼠模型可用于研究人胆脂瘤的发生发展。我们的结果支持这样一种观点，即胆脂瘤可能起源于鼓膜或外耳道表皮迁移的过度角化细胞。
