5-甲氧基补骨脂素与人血清低密度脂蛋白的结合

Binding of 5-methoxypsoralen to human serum low density lipoproteins.

作者信息

Melo T de S, Morlière P, Goldstein S, Santus R, Dubertret L, Lagrange D

出版信息

Biochem Biophys Res Commun. 1984 Apr 30;120(2):670-6. doi: 10.1016/0006-291x(84)91308-1.

Abstract

5-methoxypsoralen (5-MOP) binds to human serum low density lipoproteins (LDL) according to a two-step process. Scatchard analysis of the first step yields K = 1.4 X 10(5) M-1 and 4 binding sites. It involves the LDL apoprotein. The second step corresponds to a solubilization, in the lipidic core, of congruent to 45 molecules of 5MOP per LDL molecule. It is accompanied by a large blue shift of the 5MOP fluorescence. The ability of LDL to bind 5MOP and to carry it into various cells may explain some biological effects sometimes encountered during PUVA therapy.

摘要

5-甲氧基补骨脂素（5-MOP）通过两步过程与人血清低密度脂蛋白（LDL）结合。第一步的Scatchard分析得出K = 1.4×10⁵ M⁻¹和4个结合位点。这一步涉及LDL载脂蛋白。第二步对应于每个LDL分子在脂质核心中溶解约45个5MOP分子。这伴随着5MOP荧光的大幅蓝移。LDL结合5MOP并将其携带到各种细胞中的能力可能解释了PUVA治疗期间有时会遇到的一些生物学效应。