Morikawa N, Nakayama R, Holten D
Biochem Biophys Res Commun. 1984 May 16;120(3):1022-9. doi: 10.1016/s0006-291x(84)80209-0.
The effect of dietary carbohydrate on rat liver glucose-6-phosphate dehydrogenase synthesis has been determined by using a method which can accurately quantitate relative rates of synthesis as low as 0.001 percent of total protein synthesis. Hepatocytes were incubated with (3H) leucine for 60 min and G6PD was separated from all other proteins by immunoprecipitation and electrophoresis on two-dimensional O'Farrell gels. The relative rate of synthesis of G6PD increased 70-fold (from 0.0015 to 0.11% of total protein synthesis) in hepatocytes from fasted rats refed a high carbohydrate diet. We have concluded that the 20-30 fold dietary induction of G6PD is due to a 70 and 3 fold increase in synthesis and degradation, respectively.
通过使用一种能够精确测定低至总蛋白质合成0.001%的相对合成速率的方法，已确定了膳食碳水化合物对大鼠肝脏葡萄糖-6-磷酸脱氢酶合成的影响。将肝细胞与（3H）亮氨酸孵育60分钟，然后通过免疫沉淀和在二维奥法雷尔凝胶上进行电泳，将葡萄糖-6-磷酸脱氢酶（G6PD）与所有其他蛋白质分离。在重新喂食高碳水化合物饮食的禁食大鼠的肝细胞中，G6PD的相对合成速率增加了70倍（从总蛋白质合成的0.0015%增至0.11%）。我们得出的结论是，膳食对G6PD的诱导作用提高了20至30倍，这分别是由于合成增加了70倍和降解增加了3倍所致。
