King I A, Pope F M
Biochem Biophys Res Commun. 1984 May 31;121(1):364-71. doi: 10.1016/0006-291x(84)90731-9.
All-trans retinoic acid increased the incorporation of D-[3H]galactose into particulate and soluble glycoproteins in the epidermis of cultured pig skin slices nearly two-fold. Increased incorporation of D-[3H]galactose was not blocked by tunicamycin. This effect was specific for D-[3H]galactose since the incorporation of D-[3H]glucosamine and L-[14C]leucine into epidermal glycoproteins was unaffected by all-trans retinoic acid. All-trans retinoic acid and 13-cis retinoic acid had quantitatively similar effects on D-[3H]galactose incorporation. All-trans retinyl acetate and an aromatic retinoic acid analogue ('Etretinate') were less effective. SDS polyacrylamide gel electrophoresis and fluorography showed increased incorporation of D-[3H]galactose into all epidermal glycoproteins in the presence of all-trans retinoic acid. There was no evidence for synthesis of new glycoproteins such as mucins.
全反式维甲酸使培养的猪皮肤切片表皮中D-[3H]半乳糖掺入颗粒性和可溶性糖蛋白的量增加了近两倍。衣霉素不会阻止D-[3H]半乳糖掺入量的增加。这种效应是D-[3H]半乳糖所特有的，因为D-[3H]葡萄糖胺和L-[14C]亮氨酸掺入表皮糖蛋白的过程不受全反式维甲酸的影响。全反式维甲酸和13-顺式维甲酸对D-[3H]半乳糖掺入的影响在数量上相似。全反式醋酸视黄酯和一种芳香族维甲酸类似物（“依曲替酯”）的效果较差。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和荧光自显影显示，在全反式维甲酸存在的情况下，D-[3H]半乳糖掺入所有表皮糖蛋白的量增加。没有证据表明有新的糖蛋白如粘蛋白合成。
