Takano A, Shibayama Y, Nakata K
Liver. 1984 Apr;4(2):97-104. doi: 10.1111/j.1600-0676.1984.tb00913.x.
The morphogenesis of the vacuolation of liver cells caused by congestion was studied in the rat. Congestion of the liver was produced by constriction of the inferior vena cava just below the diaphragm. Vacuoles appeared within 5 min after the constriction (inferior vena cava pressure: 50 mm H2O or more). Vacuolation began as a small and saccular invagination of the sinusoidal surface of the liver cell membrane, and at the initial stage the vacuolar lumen connected with the space of Disse through a narrow slit. The small vacuoles increased in size and became larger by fusing with each other. The limiting membrane of vacuoles showed identical enzymological activities to those in the liver cell membrane. Vacuoles contained some components of blood and horseradish peroxidase injected into the femoral vein. These findings suggest that vacuolation of liver cells is formed by invaginations of liver cell membrane.
在大鼠中研究了由淤血引起的肝细胞空泡化的形态发生过程。通过在膈下紧束下腔静脉造成肝脏淤血。紧束后5分钟内出现空泡（下腔静脉压力：50 mmHg或更高）。空泡化始于肝细胞膜窦状表面的小囊状内陷，在初始阶段，空泡腔通过狭窄缝隙与狄氏间隙相连。小空泡尺寸增大并通过相互融合变得更大。空泡的界膜显示出与肝细胞膜相同的酶学活性。空泡含有注入股静脉的一些血液成分和辣根过氧化物酶。这些发现表明肝细胞空泡化是由肝细胞膜的内陷形成的。
