[染色质蛋白参与细胞核对糖皮质激素受体复合物的结合]
[Participation of chromatin proteins in the binding of glucocorticoid receptor complexes by the cell nucleus].
作者信息
Luksha G L, Romanov G A
出版信息
Nauchnye Doki Vyss Shkoly Biol Nauki. 1984(4):22-6.
The binding of the purified glucocorticoid-receptor complexes from rat liver cytosol with partially deproteinized and partially reconstituted chromatins from rats liver and brain has been investigated. The chromatin was deproteinized by stepwise extraction of nuclei with NaC1 solution of raised molarity. The partial chromatin reconstruction was accomplished by the addition of individual fractions of extracted proteins to DNA-cellulose. It has been shown that in both test-systems used some fractions of nuclear proteins in particular the fractions of loosely associated with chromatin nonhistone proteins are capable of modulating specifically (mainly positively) the binding of glucocorticoid-receptor complexes with chromatin.
对来自大鼠肝脏胞质溶胶的纯化糖皮质激素受体复合物与来自大鼠肝脏和大脑的部分脱蛋白和部分重构染色质的结合进行了研究。通过用摩尔浓度升高的氯化钠溶液逐步提取细胞核使染色质脱蛋白。通过将提取的蛋白质的各个部分添加到DNA-纤维素上来完成部分染色质重构。结果表明，在所用的两个测试系统中，某些核蛋白部分，特别是与染色质非组蛋白松散结合的部分，能够特异性地（主要是正向地）调节糖皮质激素受体复合物与染色质的结合。
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