Rothwell N J, Saville M E, Stock M J
Biosci Rep. 1984 Apr;4(4):351-7. doi: 10.1007/BF01140499.
Four days of fasting in the rat reduced brown-adipose-tissue (BAT) mass, mitochondrial protein, and tissue protein content. Specific binding of guanosine diposphate (GDP) to BAT mitochondria was depressed by 55% in 4d-fasted rats. Rats fasted for 3 d, and then refed a single carbohydrate meal (40 kJ), showed a significant increase in specific GDP-binding (27% above fasted) 24 h later, and a large increase in total binding. Specific activities of cytochrome oxidase and alpha-glycerophosphate dehydrogenase in BAT mitochondria were not significantly affected by fasting or refeeding. These results suggest that BAT may be partly responsible for the fall in metabolic rate associated with fasting and the delayed increase after carbohydrate refeeding. These effects may be due to changes in the mitochondrial proton-conductance pathway in brown fat.
大鼠禁食四天会使棕色脂肪组织（BAT）的质量、线粒体蛋白和组织蛋白含量降低。在禁食4天的大鼠中，二磷酸鸟苷（GDP）与BAT线粒体的特异性结合降低了55%。禁食3天的大鼠，然后喂食一顿单一碳水化合物餐（40千焦），24小时后特异性GDP结合显著增加（比禁食时高27%），总结合也大幅增加。BAT线粒体中细胞色素氧化酶和α-甘油磷酸脱氢酶的比活性不受禁食或再喂食的显著影响。这些结果表明，BAT可能部分导致了与禁食相关的代谢率下降以及碳水化合物再喂食后代谢率的延迟增加。这些影响可能是由于棕色脂肪中线粒体质子传导途径的变化所致。
