一种与鸟类和爬行类δ晶体蛋白相关的七鳃鳗晶状体蛋白。

A lamprey lens protein related to avian and reptilian delta crystallin.

作者信息

de Pomerai D I, Ellis D K, Carr A

出版信息

Curr Eye Res. 1984 May;3(5):729-35. doi: 10.3109/02713688409065595.

Abstract

Delta crystallin has hitherto been considered specific to reptiles and birds. Evidence presented in this paper suggests that a major lens protein in adult river lampreys (Lampetra fluviatilis) is related to chick delta crystallin. Both are of similar size (Mr 45-50,000), and the lamprey protein cross-reacts with antibodies against chick delta crystallin in immunodiffusion, immunoelectrophoretic and immunoblotting tests. However, the charge properties and V8 proteolytic fragment patterns of the lamprey protein are markedly different from those of avian and reptilian delta crystallins. Preliminary evidence on the occurrence of delta-related DNA sequences in the genomes of all vertebrates examined, is also discussed.

摘要

迄今为止，δ-晶体蛋白一直被认为是爬行动物和鸟类所特有的。本文所提供的证据表明，成年河七鳃鳗（Lampetra fluviatilis）晶状体中的一种主要蛋白质与鸡的δ-晶体蛋白相关。两者大小相似（分子量为45,000 - 50,000），并且在免疫扩散、免疫电泳和免疫印迹试验中，七鳃鳗蛋白能与抗鸡δ-晶体蛋白的抗体发生交叉反应。然而，七鳃鳗蛋白的电荷特性和V8蛋白酶解片段模式与鸟类和爬行动物的δ-晶体蛋白明显不同。本文还讨论了在所有检测的脊椎动物基因组中存在δ-相关DNA序列的初步证据。