Karim S A, de Pomerai D I
Department of Biology, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.
Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):79-83. doi: 10.1042/bj2850079.
Chick-embryo neuroretinal cells convert extensively into lens under low-glucose conditions, but this transdifferentiation process is blocked by high-glucose media. We have previously observed an inverse relationship between the levels of glycogen (a marker of normal retinoglial differentiation) and of delta-crystallin (a lens marker) in such cultures. However, most of the glycogen accumulated under high-glucose conditions is apparently localized in those glial (G) cells underlying clusters of neurons (N cells). We here show that glial-enriched cultures (largely depleted of N cells) both accumulate glycogen and fail to transdifferentiate in high-glucose media. Moreover, glycogen localization in groups of glial cells is unaffected by the absence of N cells. Thus the choice between normal and foreign differentiation pathways is made autonomously within the retinoglial-cell population and is not influenced significantly by the presence or absence of N cells.
在低糖条件下，鸡胚神经视网膜细胞会大量转化为晶状体，但这种转分化过程会被高糖培养基阻断。我们之前在此类培养物中观察到糖原（正常视网膜神经胶质分化的标志物）水平与δ-晶状体蛋白（晶状体标志物）水平呈负相关。然而，在高糖条件下积累的大部分糖原显然位于神经元簇（N细胞）下方的那些神经胶质（G）细胞中。我们在此表明，富含神经胶质的培养物（基本上不含N细胞）在高糖培养基中既会积累糖原，也不会发生转分化。此外，神经胶质细胞群中糖原的定位不受N细胞缺失的影响。因此，在正常和异常分化途径之间的选择是在视网膜神经胶质细胞群体内自主做出的，并且不受N细胞存在与否的显著影响。
