Synaptosomal [3H]GABA uptake and [3H]nipecotic acid binding in audiogenic seizure susceptible (DBA/2) and resistant (C57 B1/6) mice.

The kinetics of [3H]GABA uptake into synaptosomes prepared from the cerebral cortex, cerebellum and pons-medulla of DBA/2 (audiogenic seizure susceptible) and C57 B1/6 (audiogenic seizure resistant) mice were determined at various ages. Vmax of uptake decreased with age in all regions of both strains, Km was unchanged. There were no regional differences in the Vmax between strains at any age but Km was lower in DBA/2 mice at 21-23 days in cerebellum and at 40-43 days in pons-medulla. There were no strain differences in the binding of [3H]nipecotic acid to crude cortical membrane fractions at any age. The age related audiogenic seizure susceptibility of DBA/2 mice is not related to an abnormality of neuronal GABA uptake.