Burns B G, Sangalang G B, Freeman H C, McMenemy M
Gen Comp Endocrinol. 1984 Jun;54(3):422-8. doi: 10.1016/0016-6480(84)90157-6.
Lobster testes have been demonstrated to contain steroid 20-ketone reductase by their capacity to convert [14C]progesterone to 20 alpha-dihydroprogesterone (20 alpha-DHP). The major product was isopolar and identical with 20 alpha-DHP during thin-layer chromatography, high-pressure liquid chromatography, mass spectrometry, and acetate derivative formation. The vas deferens from the lobster was also capable of the same conversions but to a lesser extent. Lobster testes converted [14C]pregnenolone to a major product identified as 20-dihydropregnenolone (20-DHPe) by mass spectrometry after purification by thin-layer chromatography and high-pressure liquid chromatography. The presence of delta 5,3 beta-ol dehydrogenase and delta 5,delta 4-isomerase in the lobster were also indicated. [14C]Cholesterol was not transformed to steroid hormones by lobster testes under the same experimental conditions.
龙虾睾丸已被证明含有类固醇20-酮还原酶，因为它们能够将[14C]孕酮转化为20α-二氢孕酮（20α-DHP）。在薄层色谱、高压液相色谱、质谱分析以及醋酸酯衍生物形成过程中，主要产物的极性与20α-DHP相同且与之相同。龙虾的输精管也能够进行相同的转化，但程度较小。通过薄层色谱和高压液相色谱纯化后，经质谱分析，龙虾睾丸将[14C]孕烯醇酮转化为一种主要产物，鉴定为20-二氢孕烯醇酮（20-DHPe）。还表明龙虾中存在δ5,3β-醇脱氢酶和δ5,δ4-异构酶。在相同实验条件下，龙虾睾丸未将[14C]胆固醇转化为类固醇激素。
