Coran A G, Strodel W E, Heidelberger K
Int Surg. 1984 Jan-Mar;69(1):81-4.
In order to eliminate the mucosal dissection of the endorectal procedure, a puppy model was developed in which the rectal mucosa was treated with either 5% phenol or 1N sodium hydroxide prior to pulling the proximal colon through and performing an anal anastomosis. The phenol and sodium hydroxide were neutralized respectively with 70% isopropyl alcohol and 1% acetic acid to prevent injury to the pulled-through bowel. Six puppies were treated in each group. All dogs survived the study and were able to return to normal diets. The dogs were sacrificed at one, two and three-week intervals after operation. Microscopic examination of the rectum demonstrated that, in the group treated with sodium hydroxide, the mucosa was destroyed and was replaced with a granulating layer which was adherent to the pulled-through colonic serosa. The group treated with phenol did not show the same degree of mucosal destruction and, in these animals, the pulled-through bowel was not adherent to the rectum. This study suggests that, with refinement, chemical destruction of the rectal mucosa may be a further beneficial modification of the endorectal procedure.
为了消除经直肠手术中的黏膜剥离，建立了一个幼犬模型，即在将近端结肠牵出并进行肛门吻合之前，先用5%苯酚或1N氢氧化钠处理直肠黏膜。分别用70%异丙醇和1%乙酸中和苯酚和氢氧化钠，以防止对牵出的肠管造成损伤。每组治疗6只幼犬。所有犬只均存活至研究结束，并能够恢复正常饮食。术后每隔1周、2周和3周处死犬只。直肠的显微镜检查显示，在氢氧化钠治疗组中，黏膜被破坏，取而代之的是一层肉芽组织层，该层附着于牵出的结肠浆膜。苯酚治疗组未表现出相同程度的黏膜破坏，在这些动物中，牵出的肠管未与直肠粘连。本研究表明，经过改进，直肠黏膜的化学破坏可能是经直肠手术的进一步有益改进。
