Turner M S, Dawson W O
Intervirology. 1984;21(4):224-8. doi: 10.1159/000149524.
The actinomycin-D(AMD)-sensitive step of replication of three plant viruses - cowpea chlorotic mottle virus (CCMV), cowpea mosaic virus, and the cowpea strain of tobacco mosaic virus - in cowpea was specific for each virus. Different combinations of the viruses were examined in which leaves were inoculated with one virus and incubated for 48 h until multiplication was almost resistant to AMD. At that time, the same leaves were inoculated with a different virus and the ability of AMD to inhibit multiplication of the second virus was determined at intervals afterwards. In all combinations tested, the first virus had no effect on the sensitivity of the second virus to AMD. Also, resistance to AMD, which appeared to spread from cell to cell in advance of the virus in mechanically inoculated leaves, did not spread appreciably faster than CCMV into systemically infected upper leaves.
放线菌素D（AMD）对三种植物病毒——豇豆褪绿斑驳病毒（CCMV）、豇豆花叶病毒和烟草花叶病毒豇豆株系——在豇豆中复制的敏感阶段，对每种病毒而言都是特定的。对不同病毒组合进行了检测，在这些组合中，叶片先接种一种病毒，并培养48小时，直至增殖几乎对AMD产生抗性。此时，用另一种不同的病毒接种相同的叶片，之后定期测定AMD抑制第二种病毒增殖的能力。在所有测试的组合中，第一种病毒对第二种病毒对AMD的敏感性没有影响。此外，在机械接种的叶片中，对AMD的抗性似乎在病毒之前从一个细胞扩散到另一个细胞，其扩散到系统感染的上部叶片中的速度并不比CCMV明显快。
