Raineri M
Histochem J. 1984 Jun;16(6):601-16. doi: 10.1007/BF01003388.
Cholinesterase activity has been investigated in Rotifera Bdelloidea (Philodina roseola, Philodina tubercolata, Rotaria rotatoria and other unidentified species) by histochemical methods and in vivo observations. Parallel histological studies have been carried out. The enzyme specificity was tested by employing different substrates and inhibitors. The effects in vivo of tubocurarin, bungarotoxin and acetylcholine were also observed. Acetylcholinesterase activity is localized in the nervous and muscular tissues, in sensory organs and in all the ciliated cells. Secretory cells (subcerebral, salivary and pedal glands) and gonad cells (nuclei of the syncytial vitellarium and follicular layer, oocytes and eggs) show both acetyl- and butyrylcholinesterase activities. The effects in vivo of cholinesterase inhibitors, as well as those of tubocurarin, bungarotoxin and acetylcholine, are consistent with the histochemical results, indicating a cholinergic system of transmission and acetylcholinesterase, as well as butyrylcholinesterase, activity.
已通过组织化学方法和体内观察对蛭形轮虫（玫瑰旋轮虫、瘤突旋轮虫、旋轮虫及其他未鉴定物种）的胆碱酯酶活性进行了研究。同时开展了平行的组织学研究。通过使用不同的底物和抑制剂来测试酶的特异性。还观察了筒箭毒碱、银环蛇毒素和乙酰胆碱在体内的作用。乙酰胆碱酯酶活性定位于神经和肌肉组织、感觉器官以及所有纤毛细胞中。分泌细胞（脑下腺、唾液腺和足腺）和性腺细胞（合胞卵黄腺和滤泡层的细胞核、卵母细胞和卵子）同时显示出乙酰胆碱酯酶和丁酰胆碱酯酶活性。胆碱酯酶抑制剂以及筒箭毒碱、银环蛇毒素和乙酰胆碱在体内的作用与组织化学结果一致，表明存在胆碱能传递系统以及乙酰胆碱酯酶和丁酰胆碱酯酶活性。
