Dasse K A
J Biomed Mater Res. 1984 Apr;18(4):403-11. doi: 10.1002/jbm.820180408.
Infection continues to pose the major obstacle to long-term percutaneous access. Development of methods to prevent infection or techniques to determine early onset of infection at a time when antibiotic therapy may prove successful would be of enormous value. Our laboratory has been working toward developing and testing a noninvasive semiquantitative swab culture technique (SQ) to monitor percutaneous leads for infection. This technique was found to have a 76% sensitivity having identified 47 of 62 organisms detected by a quantitative tissue culture technique (Q) at the time of system explant. Furthermore, 47 of 61 organisms identified by the SQ technique accurately detected those isolated by the Q techniques. Accordingly, the SQ technique has a 77% specificity. This technique was capable of detecting organisms a median of 14 days prior to overt clinical infection. Prompt initiation of oral antibiotic treatment based on SQ results has doubled system survival compared with untreated systems. Clearly, the SQ technique has proven useful to monitoring percutaneous devices.
感染仍然是长期经皮通路的主要障碍。开发预防感染的方法或在抗生素治疗可能成功时确定感染早期发作的技术将具有巨大价值。我们实验室一直在致力于开发和测试一种非侵入性半定量拭子培养技术（SQ），以监测经皮导线是否感染。在系统取出时，通过定量组织培养技术（Q）检测到62种微生物，该技术能够识别其中47种，灵敏度为76%。此外，SQ技术识别的61种微生物中有47种准确检测到了Q技术分离出的微生物。因此，SQ技术的特异性为77%。该技术能够在明显临床感染前中位14天检测到微生物。根据SQ结果迅速开始口服抗生素治疗，与未治疗的系统相比，系统存活率提高了一倍。显然，SQ技术已被证明对监测经皮装置很有用。
