Rubin R W, Lyubkin A K, Pressman B C
J Cell Biol. 1984 Jul;99(1 Pt 1):356-60. doi: 10.1083/jcb.99.1.356.
A two-dimensional polyacrylamide gel analysis of three types of bovine exocytotic granule membranes has been undertaken. Great care was taken to purify the membranes of biochemical homogeneity with minimal contamination from other membrane sources. The goal was to identify proteins that were present in all three membrane types. Although a number of minor components were observed that co-migrated for two membrane types, no proteins were detected that were present in all three granule membranes. We therefore conclude that such exocytosis-specific proteins do not exist or that they represent less than 0.1% of the total membrane protein present in a given isolated membrane preparation.
对三种类型的牛分泌颗粒膜进行了二维聚丙烯酰胺凝胶分析。在纯化膜时格外小心，以确保其生化同质性，同时尽量减少来自其他膜源的污染。目的是鉴定在所有三种膜类型中都存在的蛋白质。虽然观察到一些次要成分在两种膜类型中共迁移，但未检测到在所有三种颗粒膜中都存在的蛋白质。因此，我们得出结论，这种特异性的胞吐作用蛋白质不存在，或者它们在给定的分离膜制剂中占总膜蛋白的比例不到0.1%。
