Schweitzer E S, Kelly R B
J Cell Biol. 1985 Aug;101(2):667-76. doi: 10.1083/jcb.101.2.667.
We have introduced the gene for human growth hormone (hGH) into PC12 cells, a rat pheochromocytoma-derived cell line with neuronal characteristics, and have isolated stable cell lines that express this protein. hGH is stored within the cells in membrane-bounded vesicles that are indistinguishable from the endogenous catecholaminergic synaptic vesicles. When the transfected cells are stimulated by carbachol or direct depolarization, they release norepinephrine and hGH with parallel kinetics. Treatment of the transfected cells with nerve growth factor results in a twofold increase in the amounts of hGH stored in and secreted from the cells. Not all proteins are packaged into the synaptic vesicles, since the rate of release of laminin, a soluble secreted protein endogenous to PC12 cells, is not stimulated by carbachol. This neuronal cell line therefore possesses at least two distinct pathways for secretion and can selectively package a foreign endocrine hormone into the regulated pathway.
我们已将人生长激素（hGH）基因导入PC12细胞，这是一种源自大鼠嗜铬细胞瘤且具有神经元特性的细胞系，并分离出了表达该蛋白的稳定细胞系。hGH存储在细胞内有膜包被的囊泡中，这些囊泡与内源性儿茶酚胺能突触囊泡无法区分。当用卡巴胆碱刺激转染细胞或直接使其去极化时，它们会以平行动力学释放去甲肾上腺素和hGH。用神经生长因子处理转染细胞会使细胞内存储和分泌的hGH量增加两倍。并非所有蛋白质都会被包装到突触囊泡中，因为层粘连蛋白（PC12细胞内源性可溶性分泌蛋白）的释放速率不受卡巴胆碱刺激。因此，这种神经元细胞系至少具有两种不同的分泌途径，并且能够将外源内分泌激素选择性地包装到调节途径中。
