Galli Kienle M, Galli G, Bosisio E, Cighetti G, Paoletti R
J Chromatogr. 1984 Apr 27;289:267-76. doi: 10.1016/s0021-9673(00)95094-5.
Methods are described for the evaluation of HMGCoA reductase and cholesterol 7 alpha-hydroxylase activities. The methods are based on the measurement by selected-ion monitoring of mevalonate, formed from HMGCoA and of 7 alpha-hydroxycholesterol formed from cholesterol, respectively. The methods are as sensitive as those based on the use of radioisotopes but less time-consuming because quantitation is carried out on total lipid extracts. In the case of cholesterol 7 alpha-hydroxylase, the procedure does not require the use of exogenous cholesterol as the substrate, so problems related to its equilibration with endogenous microsomal cholesterol are avoided.
描述了评估HMGCoA还原酶和胆固醇7α-羟化酶活性的方法。这些方法分别基于通过选择离子监测由HMGCoA形成的甲羟戊酸和由胆固醇形成的7α-羟基胆固醇的测量。这些方法与基于使用放射性同位素的方法一样灵敏，但耗时较少，因为定量是在总脂质提取物上进行的。对于胆固醇7α-羟化酶，该程序不需要使用外源性胆固醇作为底物，因此避免了与内源性微粒体胆固醇平衡相关的问题。
