Hauffe S A, Dubois J P
J Chromatogr. 1984 May 4;290:223-30. doi: 10.1016/s0021-9673(01)93577-0.
A high-performance liquid chromatographic method is described for the determination of cadralazine in human plasma and urine. To 1 g of plasma (pH 7) or urine (adjusted to pH 11), internal standard was added and the samples were extracted with chloroform-ethanol (95:5, v/v). The substances were then back-extracted into acid (pH 2) and 100 microliter of the aqueous phase were injected. Chromatography was performed on a 10-micron LiChrosorb RP-8 column with acetonitrile-phosphate buffer pH 6 (15:85, v/v) as eluent at a flow-rate of 2.7 ml/min. The substances were detected by UV spectrophotometry at 254 nm. Concentrations down to 0.141 nmol/g in plasma or 10.59 nmol/g in urine could be measured with very good precision. This method was applied to samples from two healthy volunteers given a single oral dose of 10 mg or 20 mg of cadralazine .
本文描述了一种高效液相色谱法，用于测定人血浆和尿液中的卡屈嗪。向1 g血浆（pH 7）或尿液（调至pH 11）中加入内标，样品用氯仿 - 乙醇（95:5，v/v）萃取。然后将物质反萃取到酸（pH 2）中，并注入100微升水相。在10微米的LiChrosorb RP - 8柱上进行色谱分析，以乙腈 - pH 6的磷酸盐缓冲液（15:85，v/v）作为洗脱剂，流速为2.7 ml/min。通过紫外分光光度法在254 nm处检测这些物质。血浆中低至0.141 nmol/g或尿液中低至10.59 nmol/g的浓度都能以非常高的精密度进行测定。该方法应用于两名健康志愿者单次口服10 mg或20 mg卡屈嗪后的样品。
