从潜在代谢产物和降解产物中高效液相色谱分离肼屈嗪。人血浆和尿液中该药物的定量分析。

High-performance liquid chromatographic separation of cadralazine from its potential metabolites and degradation products. Quantitation of the drug in human plasma and urine.

作者信息

Crolla T, Santini F, Visconti M, Pifferi G

出版信息

J Chromatogr. 1984 Sep 14;310(1):139-49. doi: 10.1016/0378-4347(84)80075-4.

Abstract

The chromatographic behaviour of cadralazine and its potential metabolites and degradation products with respect to pH, buffer molarity and composition of eluent is described. A selective method with an adequate sensitivity for the determination of the drug in human plasma and urine is also reported. The method includes extraction of biological fluids with chloroform and the analysis of extracts on a reversed-phase column with isocratic elution and detection at 254 nm. The method has been applied to the analysis of plasma and urine of a patient administered a single oral dose of 30 mg of cadralazine.

摘要

描述了卡屈嗪及其潜在代谢物和降解产物在不同pH值、缓冲液摩尔浓度和洗脱液组成条件下的色谱行为。还报道了一种用于测定人血浆和尿液中该药物的具有足够灵敏度的选择性方法。该方法包括用氯仿萃取生物流体，并在反相柱上采用等度洗脱，于254 nm波长处进行检测，分析萃取物。该方法已应用于一名单次口服30 mg卡屈嗪患者的血浆和尿液分析。