Frappart L, Agard C, Lin H C, Grimaud J A, Palayer C, Rochet Y, Brémond A
J Gynecol Obstet Biol Reprod (Paris). 1984;13(2):117-23.
Thirteen ovarian tumours were the subject of a histochemical and immunohistochemical study using antibodies to collagen IV, III, I, and to laminin. Our study shows that it is not possible to assess the integrity of the basement membrane using simple histochemical methods (P.A.S., Gordon Sweets staining). In contrast, in benign epithelial tumours, a continuous basement membrane may be visualized beneath the epithelium of the cysts using antibodies to collagen IV and to laminin, collagen IV and laminin being two of the constituents of basement membranes. In malignant epithelial tumours, the immunoreactive material of collagen IV type or of laminin type, is arranged in a distinctly discontinuous manner, and is of unequal thickness around the edge of the cysts or of the neoplastic clusters. In three cases of borderline epithelial tumours, the discontinuity of the immunoreactive material (collagen IV or laminin) lying beneath the epithelium of the cysts, is not so pronounced. In comparison with studies carried out on the cervix uteri, this latter type of tumor, because of the constitution of its basement membranes, would appear to occur at a particular moment in their evolution, i.e. when they pass from the intra-epithelial stage and become infiltrative.
13例卵巢肿瘤接受了一项组织化学和免疫组织化学研究，该研究使用了针对IV型、III型、I型胶原蛋白以及层粘连蛋白的抗体。我们的研究表明，使用简单的组织化学方法（过碘酸雪夫反应、戈登-斯威茨染色）无法评估基底膜的完整性。相比之下，在良性上皮性肿瘤中，使用针对IV型胶原蛋白和层粘连蛋白的抗体，可在囊肿上皮下方看到连续的基底膜，IV型胶原蛋白和层粘连蛋白是基底膜的两种成分。在恶性上皮性肿瘤中，IV型胶原蛋白或层粘连蛋白类型的免疫反应性物质排列明显不连续，且在囊肿边缘或肿瘤细胞簇周围厚度不均。在3例交界性上皮性肿瘤中，位于囊肿上皮下方的免疫反应性物质（IV型胶原蛋白或层粘连蛋白）的不连续性不那么明显。与对子宫颈进行的研究相比，后一种类型的肿瘤由于其基底膜的构成，似乎在其演变的特定时刻发生，即当它们从上皮内阶段转变为浸润性阶段时。
