Patel R, Brown J C
J Immunol Methods. 1984 Jul 6;71(2):211-5. doi: 10.1016/0022-1759(84)90067-x.
A simple, economical, and time-saving procedure for freezing freshly prepared hybridomas at--70 degrees C is described. The procedure allows for supernatant fluid collection and storage of hybridomas in 96-well flexible polyvinyl microtiter plates. As the plates are sealed, and the plate is relatively soft, individual wells can be removed and cells recovered at the investigator's discretion. Consequently, this procedure allows significant latitude with regard to the time-consuming steps usually associated with hybridoma screening, expansion, and sub-cloning techniques.
本文描述了一种简单、经济且省时的将新制备的杂交瘤细胞在-70℃下冻存的方法。该方法允许收集上清液，并将杂交瘤细胞储存在96孔柔性聚乙烯微量滴定板中。由于板已密封，且板相对较软，研究人员可根据需要移除单个孔并回收细胞。因此，该方法在通常与杂交瘤筛选、扩增和亚克隆技术相关的耗时步骤方面具有很大的灵活性。
