含有蓖麻毒素A链的细胞反应性免疫毒素的细胞毒性可被含有蓖麻毒素B链的抗免疫毒素增强。
Cytotoxicity of a cell-reactive immunotoxin containing ricin A chain is potentiated by an anti-immunotoxin containing ricin B chain.
作者信息
Vitetta E S, Fulton R J, Uhr J W
出版信息
J Exp Med. 1984 Jul 1;160(1):341-6. doi: 10.1084/jem.160.1.341.
Abstract
In vitro killing of the human Daudi cell line by either univalent [F(ab')] or divalent (IgG) forms of rabbit anti-human Ig (RAHIg) coupled to ricin A chain can be specifically potentiated by a "piggyback" treatment with ricin B chain coupled to goat anti-rabbit Ig (GARIg). When cells are treated with univalent immunotoxin (IT) [F(ab') RAHIg-A] and then cultured, IT can be detected on the cell surface for at least 5 h, since GARIg-B can still enhance killing at this time. These results provide a strategy for in vivo use of A chain- and B chain-containing IT.
摘要
与蓖麻毒素A链偶联的单价[F(ab')]或二价（IgG）形式的兔抗人Ig（RAHIg）对人Daudi细胞系的体外杀伤作用，可通过与山羊抗兔Ig（GARIg）偶联的蓖麻毒素B链进行“搭载”处理而得到特异性增强。当细胞用单价免疫毒素（IT）[F(ab') RAHIg-A]处理后再进行培养时，IT可在细胞表面检测到至少5小时，因为此时GARIg-B仍能增强杀伤作用。这些结果为含A链和B链的IT的体内应用提供了一种策略。
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