Wilson S K, Heptinstall R H
Kidney Int. 1984 Mar;25(3):492-501. doi: 10.1038/ki.1984.45.
A perfusion-fixation and vascular casting technique was used to assess the effects of acute, angiotensin-induced hypertension on the intrarenal arteries and, for comparison, the small arteries of the intestine. The first objective was to establish that the technique accurately preserves postmortem the vascular changes induced by acute hypertension. To do this, the easily accessible intestinal arteries were examined and photographed both in vivo and after fixation and injection of Batson's no. 17 casting resin in a group of angiotensin-treated rats and controls. The second objective was to apply the technique to observe and compare acute hypertensive changes in the intrarenal and intestinal arteries; studies included scanning electron microscopy of vascular casts and transmission electron microscopy of vessel walls using ferritin as a tracer to assess permeability. In the angiotensin-treated rats, casts of both intrarenal and intestinal arteries showed many well-defined zones of constriction and nonconstriction. Transmission electron microscopy of both the smaller intrarenal (interlobular) arteries and intestinal vessels revealed focal smooth muscle rarefaction and abnormal permeability to ferritin, found only in the nonconstricted zones. This study provides new evidence that in the kidney, as in the intestine, acute hypertension produces a characteristic pattern of arterial constriction and nonconstriction, and that hypertensive vascular lesions with accompanying increased permeability occur exclusively in the nonconstricted zones.
采用灌注固定和血管铸型技术，评估急性血管紧张素诱导的高血压对肾内动脉的影响，并与肠小动脉进行比较。第一个目的是确定该技术能准确地在死后保存急性高血压引起的血管变化。为此，在一组接受血管紧张素治疗的大鼠和对照组中，对易于获取的肠动脉在体内以及在固定和注射巴特森17号铸型树脂后进行检查和拍照。第二个目的是应用该技术观察和比较肾内动脉和肠动脉的急性高血压变化；研究包括对血管铸型进行扫描电子显微镜检查，以及使用铁蛋白作为示踪剂对血管壁进行透射电子显微镜检查以评估通透性。在接受血管紧张素治疗的大鼠中，肾内动脉和肠动脉的铸型均显示出许多界限分明的收缩区和非收缩区。对较小的肾内（小叶间）动脉和肠血管进行透射电子显微镜检查发现，仅在非收缩区出现局灶性平滑肌稀疏和对铁蛋白的异常通透性。这项研究提供了新的证据，即在肾脏中，如同在肠道中一样，急性高血压会产生特征性的动脉收缩和非收缩模式，并且伴有通透性增加的高血压血管病变仅发生在非收缩区。
