转运RNA核苷酸转移酶与Affi-Gel Blue的结合：酶的快速纯化及结合研究
Binding of tRNA nucleotidyltransferase to Affi-Gel Blue: rapid purification of the enzyme and binding studies.
作者信息
Deutscher M P, Masiakowski P
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1947-54. doi: 10.1093/nar/5.6.1947.
Abstract
Rabbit liver tRNA nucleotidyldransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.
摘要
兔肝tRNA核苷酸转移酶能与Affi-Gel Blue柱结合，并能用tRNA特异性洗脱。这一发现促使人们开发了一种快速纯化该酶的方法。这种吸附剂还被用于评估tRNA核苷酸转移酶与各种多核苷酸和底物的相互作用。低浓度的tRNA-C-C能有效地从Affi-Gel Blue上解吸出该酶，tRNA-C-C-A的效果稍差，而poly(A)、poly(C)、ATP或CTP则完全不能解吸该酶。
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Isolation and properties of tRNA nucleotidyl transferase from yeast.酵母中tRNA核苷酸转移酶的分离与性质
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