一种纯化限制性内切酶的通用方法。
A general method for the purification of restriction enzymes.
作者信息
Greene P J, Heyneker H L, Bolivar F, Rodriguez R L, Betlach M C, Covarrubias A A, Backman K, Russel D J, Tait R, Boyer H W
出版信息
Nucleic Acids Res. 1978 Jul;5(7):2373-80. doi: 10.1093/nar/5.7.2373.
Abstract
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
摘要
已开发出一种简化的限制性内切酶纯化方法。该方法采用磷酸纤维素和羟基磷灰石层析，得到的酶纯度足以用于DNA测序、分子克隆和物理图谱绘制。
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本文引用的文献
1
Restriction endonucleases.限制性内切核酸酶
CRC Crit Rev Biochem. 1976 Nov;4(2):123-64. doi: 10.3109/10409237609105456.
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Restriction endonucleases in the analysis and restructuring of dna molecules.用于DNA分子分析和重组的限制性核酸内切酶。
Annu Rev Biochem. 1975;44:273-93. doi: 10.1146/annurev.bi.44.070175.001421.
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