Johnson R M
Muscle Nerve. 1984 Jun;7(5):369-73. doi: 10.1002/mus.880070505.
Membrane protein phosphorylation has been measured in both normal and dystrophic (BIO 53.58) hamsters. After radiolabeling intact erythrocytes with exogenous 32Pi, band 3 polypeptides from dystrophic hamsters had increased incorporation of 32P, compared to age-matched controls. This was verified by membrane protein fractionation. The use of intact cell phosphorylation avoids many of the sources of experimental variability noted in experiments with isolated membranes. This work, using an animal model for the disease, supports the hypothesis of a generalized membrane defect in the muscular dystrophies.
已对正常和患肌营养不良症（BIO 53.58）的仓鼠的膜蛋白磷酸化进行了测量。在用外源性32P对完整红细胞进行放射性标记后，与年龄匹配的对照相比，患肌营养不良症仓鼠的带3多肽有更多的32P掺入。这通过膜蛋白分级分离得到了验证。使用完整细胞磷酸化避免了在分离膜实验中提到的许多实验变异性来源。这项使用该疾病动物模型的研究支持了肌营养不良症中存在普遍膜缺陷的假说。
